Galectin 3 Antikörper (Biotin)

Produktnummer ABIN114276

Produktdetails anti-Galectin 3 Antikörper (Biotin)

Target: Galectin 3 (LGALS3)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Galectin 3 Antikörper ist konjugiert mit Biotin

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Immunogen: Plasma membrane glycoproteins from C57BL/6 mouse thioglycollateelicited peritoneal exudate

Klon: M3-38

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ABIN114276 can be used for Indirect Immunofluorescence staining, including flow cytometricanalysis of live cells. The addition of propidium iodide is optional, its use eliminatesstaining artifacts caused by dead cells (1). This clone is also suitable for frozen sections(4,5) and ELISA (6).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 2 µg* of ABIN114276 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a dilution recommended by themanufacturer. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA Grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in alqiuots) at -20 °C.

Details zu Galectin 3

Target: Galectin 3 (LGALS3)

Andere Bezeichnung: Galectin-3 (LGALS3 Produkte)

Synonyme: CBP 35 antikoerper, Gal-3 antikoerper, Galectin-3 antikoerper, leg3 antikoerper, galactoside-binding antikoerper, galectin-3 antikoerper, lectin antikoerper, soluble antikoerper, CBP35 antikoerper, GAL3 antikoerper, GALBP antikoerper, GALIG antikoerper, L31 antikoerper, LGALS2 antikoerper, MAC2 antikoerper, MGC52947 antikoerper, MGC75803 antikoerper, LGALS3 antikoerper, gal-3 antikoerper, GBP antikoerper, L-34 antikoerper, Mac-2 antikoerper, gal3 antikoerper, galectin 3 antikoerper, Galectin-3 antikoerper, avian beta-defensin 3 antikoerper, lectin, galactoside-binding, soluble, 3 L homeolog antikoerper, lectin, galactoside-binding, soluble, 3 antikoerper, galectin 2 antikoerper, lectin, galactose binding, soluble 3 antikoerper, LGALS3 antikoerper, leg3 antikoerper, AvBD3 antikoerper, lgals3.L antikoerper, lgals3 antikoerper, LGALS2 antikoerper, Lgals3 antikoerper

Substanzklasse: Chemical

Hintergrund: Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.Synonyms: 35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBP, Galactose-specific lectin 3, IgE-binding protein, L-31, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Lgals3, Mac-2

Gen-ID: 10090

UniProt: P16110

Pathways: RTK Signalweg