BMP10
Reaktivität: Human
WB
Wirt: Maus
Polyclonal
unconjugated
Applikationshinweise
Peptide-ELISA: 1/50 - 1/200. In a simple ELISA using 1 g of antigen coated per well, the50 % OD was observed at an antibody dilution of 1/100. With 1 g of peptide-BSA coatedper well the 50 % OD was observed at 1/2500. Under the same conditions the end pointwas observed at 1/80K. Competitive ELISA: pep-BSA plates, coated at 1 g/well, antibodydiluted 1/10K. Pep-BSA displaces in the range 0.1 to 100 μg/mL. The free peptide does notdisplace in this assay system. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1.8 mg/mL (OD280)
Buffer
PBS, pH 7.2, with 0.09 % sodium azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
BMP10
(Bone Morphogenetic Protein 10 (BMP10))
Andere Bezeichnung
BMP10
Hintergrund
Bone morphogenetic protein-10 (BMP10) belongs to the transforming growth factor beta (TGFß) family. BMPs, named for their initial biological activity of inducing ectopic bone formation, have been linked to multiple steps of cardiac development. BMP10 is translated as a precursor protein (preproprotein) with N-terminal hydrophobic leader peptide followed by prodomain and the mature C-terminal region that is released after the cleavage at multibasic motif RXRR. Data strongly suggest that BMP10 is an essential component in modulating cardiomyocyte proliferation and maturation during cardiac ventricular development.Synonyms: BMP-10, Bone morphogenetic protein 10