Collagen Type I Antikörper

Produktnummer ABIN113983

Produktdetails anti-Collagen Type I Antikörper

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type I Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Spezifität: Human Collagen type I: 100 % Collagen type II and IV: < 0.1 % Collagen type III and V: < 1.0 % Human Fibronectin: < 0.1 %.

Kreuzreaktivität (Details): Species reactivity (expected):Cattle (< 1.0 %), Rat and Chicken (< 0.1 %).
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Purified Collagen type I from Human skin.

Anwendungsinformationen

Applikationshinweise: RIA. ELISA: >/= 1/200 (OD >/= 500). Immunflourescence Assays: 1/80. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/600. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Human skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered solution, no BSA and preservative added.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Andere Bezeichnung: Collagen Type I (COL1 Produkte)

Synonyme: collagen type III alpha 1 chain antikoerper, COL3A1 antikoerper

Hintergrund: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gen-ID: 1277

NCBI Accession: NP_000079

UniProt: P02452