Alpha-amylase 1 Antikörper

Produktnummer ABIN113204

Der Schaf Polyklonal Anti-Alpha-amylase 1-Antikörper wurde für EIA und IHC (fro) validiert. Er ist geeignet, Alpha-amylase 1 in Proben von Human und Schwein zu detektieren.

Kurzübersicht für Alpha-amylase 1 Antikörper (ABIN113204)

Target: Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))

Reaktivität: Human, Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Alpha-amylase 1 Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Alpha-amylase 1 Antikörper

Spezifität: Human salivary Amylase: 100 % Porcine pancreatic Amylase 50 % Amylase from Aspergillus oryzae 10 %

Aufreinigung: Affinity Chromatography

Immunogen: Highly pure Human Salivary Amylase.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (frozen): 1/50 - 1/200. ELISA (In a simple indirect ELISA with 400 ng of human salivary amylase bound to theplate, the 50 % OD was observed at an antibody dilution of 1/5K. Under the sameconditions, the end point was observed at 1/500K).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 5.0 mg/mL

Buffer: PBS pH 7.2 containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Alpha-amylase 1

Target: Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))

Andere Bezeichnung: alpha-Amylase 1

Hintergrund: Amylases are secreted proteins that hydrolyze 1,4 alpha glucoside bonds in oligosaccharides and polysaccharides. They catalyze the first step in digestion of dietary starch and glycogen. Alternative splicing results in multiple transcript variants encoding the same protein. Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single polypeptide chain with a molecular weight of 56 kDa (family B) or 62 kDa (family A), the difference in molecular weight being due to the presence or absence of a carbohydrate moiety. The total serum concentration of amylase is about 120g/L of which about 50 % is salivary amylase. Although salivary amylase and pancreatic amylase share 95 % amino acid sequence homology, they differ in molecular size, isoelectricpoint and antigenic properties.Synonyms: 1, 4-alpha-D-glucan glucanohydrolase 1, AMY1, AMY1A, Salivary alpha-amylase

Gen-ID: 276

NCBI Accession: NP_001008219

UniProt: P04745