This antibody recognizes Thyroglobulin (Tg), reacting with circulating Tg (Tg/T4 complex). Reacts with a different epitope to clone BGN/6E10 (AM01234PU-N) and can be used as the conjugated antibody in 2 site assay with this clone.
TG
Reaktivität: Human
IHC (p)
Wirt: Maus
Monoclonal
2H11
unconjugated
Applikationshinweise
ELISA: This antibody is suitable for use as a detection antibody in a sandwich ELISA usingABIN180965 as the Capture antibody. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This Thyroglobulin antibody was validated for use in immunohistochemistry on a panel of21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Thyroglobulin is the glycoprotein precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin is obtained from the thyroid gland and exhibits the general properties of the globulins. The human thyroglobulin (hTG) is a high molecular weight glycoprotein (605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti thyroglobulin autoantibodies were the first factor to be discovered. Anti hTG is found in all thyroid autoimmune diseases (Hashimoto's thyroiditis, Graves' diseases), with the highest level observed in Hashimoto's thyroiditis. Anti hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients.Synonyms: TGN, Thyreoglobulin