beta-2 Microglobulin Antikörper

Produktnummer ABIN112401

Produktdetails anti-beta-2 Microglobulin Antikörper

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta-2 Microglobulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Spezifität: The antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Purified Human beta-2-Microglobulin

Klon: B2M-01

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow CytometryDilution: 1 mg/mLPositive control: PBL cell line,Negative control: DAUDI human lymphoma cell line, erythrocytes. ImmunoprecipitationB2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from thelysates of human lymphoid cells. RIAThe dissociation constant of the antibody soluble beta2-Microglobulin is 1.5 x 10e-8 Mol/las determined by competitive RIA. ELISA. Immunohistochemistry (Paraffin)Dilution: 10 μg/mL. Positive tissue: kidney glomeruli. Western blotting (Reducing and Non-Reducing Conditions). Dilution: 2-4 μg/mL. Positive control: U937 human histiocytic ymphoma cell line. Negative control: EL4 mouse lymphoblastic lymphoma cell line. Sample Preparation (See Protocols).

Protokoll: Sample Preparation (Western Blotting): Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mMTris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Protease inhibitor Cocktail). Incubate60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducingLaemmli SDS-PAGE sample buffer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Andere Bezeichnung: beta-2-Microglobulin (B2M Produkte)

Synonyme: Ly-m11 antikoerper, beta2-m antikoerper, beta2m antikoerper, bwm antikoerper, wu:fa94c05 antikoerper, wu:fb10a09 antikoerper, b2m-W01 antikoerper, b2m-W03 antikoerper, b2m-Z01 antikoerper, b2m-Z02 antikoerper, b2m-Z03 antikoerper, B2M antikoerper, b2m antikoerper, B2MG antikoerper, wu:fb66f11 antikoerper, wu:fj01f08 antikoerper, zgc:64161 antikoerper, b-2-m antikoerper, beta-2-microglobulin antikoerper, beta-2 microglobulin antikoerper, beta-2-microglobulin S homeolog antikoerper, beta-2-microglobulin, like antikoerper, B2M antikoerper, B2m antikoerper, b2m antikoerper, b2m.S antikoerper, b2ml antikoerper

Hintergrund: Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin

Gen-ID: 567

NCBI Accession: NP_004039

UniProt: P61769

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process