Mitotic Cells Antikörper

Produktnummer ABIN112395

Der Maus Monoklonal anti-Mitotic Cells Antikörper (Klon 8B3G) (ABIN112395) detektiert spezifisch Mitotic Cells in FACS, IHC (fro) und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Zebrafisch (Danio rerio).

Kurzübersicht für Mitotic Cells Antikörper (ABIN112395)

Target: Mitotic Cells

Reaktivität: Human, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Klon: 8B3G

Produktdetails

Kreuzreaktivität (Details): Species reactivity (tested):Human, Zebrafish.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Total cell lysate of the human bladder carcinoma cell line T24.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: This antibody is suitable for Flow cytometric analyses (1/50-1/100) andImmunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent (1/50-1/100). Not suitable for Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the lyophilized antibody at 2-8 °C for up to one month or at -20 °C for longer.

Antigendetails

Target: Mitotic Cells

Hintergrund: The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a 'quiescent' phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase. M phase itself is highly regulated, and is divided into five stages: prophase, prometaphase, metaphase, telophase and anaphase.