CTNNA3 Antikörper (N-Term)

Produktnummer ABIN112367

Der Maus Monoklonal Anti-CTNNA3-Antikörper wurde für WB, IF, IP, IHC (p), IHC (fro) und FACS validiert. Er ist geeignet, CTNNA3 in Proben von Human zu detektieren.

Kurzübersicht für CTNNA3 Antikörper (N-Term) (ABIN112367)

Target: CTNNA3 (Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CTNNA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Klon: 892_24D2S

Produktdetails anti-CTNNA3 Antikörper

Bindungsspezifität: AA 164-177, N-Term

Spezifität: The 892_24D2S antibody against αT-Catenin does not show any cross reaction with αE-Catenin or αN-Catenin in western blot analysis. This 892_24D2S antibody reacts with an epitope located between residues 164-177 of the Human αT-Catenin protein.

Aufreinigung: Purified

Immunogen: Synthetic peptide corresponding to amino acids 164-177 of the human alphaT-catenin protein including an additional N-terminal cysteine (CVANKSDLQKTYQKL) coupled to keyhole limpet hemocyanin.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: aceton-fixed cells. Immunocytochemistry: methanol and paraformaldehyde-fixed cells, followed bypermeabilisation in 0.2 % Triton-X 100 for 2 min. Immunohistochemistry1: 1/25-1/200 with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent,Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Details zu CTNNA3

Target: CTNNA3 (Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3))

Andere Bezeichnung: Catenin alpha-3 / CTNNA3

Hintergrund: Catenins are proteins that connect cadherin/β- catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-catenins to which it shows an overall amino acid identity of 57 % . In cells transfected with αT-catenin cDNA, interaction with β-catenin was demonstrated by coimmunoprecipitation.Synonyms: Alpha Catenin 3, Alpha T-catenin, Cadherin-associated protein

Gen-ID: 29119

NCBI Accession: NP_001120856

UniProt: Q9UI47