Lamin A/C Antikörper

Produktnummer ABIN112346

Der Maus Monoklonal Anti-Lamin A/C-Antikörper wurde für WB, IF, FACS und IHC (fro) validiert. Er ist geeignet, Lamin A/C in Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Hamster und Schaf zu detektieren.

Kurzübersicht für Lamin A/C Antikörper (ABIN112346)

Target: Lamin A/C (LMNA)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Hamster, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 131C3

Produktdetails anti-Lamin A/C Antikörper

Spezifität: 131C3 reacts with an epitope located between residues 319-566 in Lamin A and C. Immunoblotting of 131C3 recognizes Nuclear Lamins A and C in Human Fibroblasts.

Aufreinigung: Purified

Immunogen: Purified Rat liver Lamins.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: 1/100-1/1000. Flow Cytometry: 1/100-1/200. Immunocytochemistry. Immunohistochemistry on Frozen Sections: 1/100-1/200 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: Lamin-A/C (LMNA)

Hintergrund: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32

Gen-ID: 4000

NCBI Accession: NP_005563

UniProt: P02545

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus