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ADRP Antikörper (N-Term)

PLIN2 Reaktivität: Human, Ratte, Hund WB, IF, IHC (p), IHC (fro) Wirt: Maus Monoclonal AP125 unconjugated
Produktnummer ABIN112185
  • Target Alle ADRP (PLIN2) Antikörper anzeigen
    ADRP (PLIN2) (Perilipin 2 (PLIN2))
    Bindungsspezifität
    • 29
    • 15
    • 8
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 5-27, N-Term
    Reaktivität
    • 115
    • 23
    • 17
    • 1
    Human, Ratte, Hund
    Wirt
    • 71
    • 40
    • 4
    • 1
    • 1
    Maus
    Klonalität
    • 66
    • 51
    Monoklonal
    Konjugat
    • 57
    • 10
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ADRP Antikörper ist unkonjugiert
    Applikation
    • 80
    • 51
    • 49
    • 36
    • 32
    • 18
    • 14
    • 14
    • 12
    • 12
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    Polypeptide Reacting: Adipophilin / ADRP, MW 48,100 (calculated from aa sequence data), apparent Mr 52,000 (after SDS-PAGE), pI 6.72. Tissue Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver, adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Tested Reactivity on Cultured Cell lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK. Negative with glioma.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human, Rat and Dog. Negative with Bovine and Mouse.
    Aufreinigung
    Affinity Chromatography
    Immunogen
    Synthetic peptide corresponding to aa 5-27 from N-terminus of Human Adipophilin.
    Klon
    AP125
    Isotyp
    IgG1
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  • Applikationshinweise
    Immunohistochemistry on Frozen Tissue: 1/10 in PBS, pH 7.4(See Ref.17 for staining protocols). Immunohistochemistry on Paraffin-Embedded Tissue (After microwave treatment). Incubate 1 h at room temperature or over night at 2-8 °C. Cytological Material. Immunoblotting (Western blot): 0.2 μg/mL using ECL has been reported for previous batchnumbers.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1.0 mL distilled water
    Buffer
    Final solution contains 0.09 % Sodium Azide and 0.5 % BSA in PBS buffer, pH 7.4
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store the lyophilized antibody at -20 °C.
  • Leitner, Hlavatý, Ertl, Gabner, Fuchs-Baumgartinger, Walter: "Lipid droplets and perilipins in canine osteosarcoma. Investigations on tumor tissue, 2D and 3D cell culture models." in: Veterinary research communications, Vol. 46, Issue 4, pp. 1175-1193, (2022) (PubMed).

    Leitner, Hlavaty, Heider, Ertl, Gabriel, Walter: "Lipid droplet dynamics in healthy and pyometra-affected canine endometrium." in: BMC veterinary research, Vol. 18, Issue 1, pp. 221, (2022) (PubMed).

    Breher-Esch, Sahini, Trincone, Wallstab, Borlak: "Genomics of lipid-laden human hepatocyte cultures enables drug target screening for the treatment of non-alcoholic fatty liver disease." in: BMC medical genomics, Vol. 11, Issue 1, pp. 111, (2019) (PubMed).

    Skwarek-Maruszewska, Hotulainen, Mattila, Lappalainen: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." in: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).

    Ohsaki, Maeda, Fujimoto: "Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins." in: Histochemistry and cell biology, Vol. 124, Issue 5, pp. 445-52, (2005) (PubMed).

  • Target
    ADRP (PLIN2) (Perilipin 2 (PLIN2))
    Andere Bezeichnung
    Adipophilin / ADFP (PLIN2 Produkte)
    Synonyme
    ADFP antikoerper, ADRP antikoerper, AA407157 antikoerper, ADPH antikoerper, Adfp antikoerper, Adrp antikoerper, adfp antikoerper, adrp antikoerper, fatvg antikoerper, MGC76047 antikoerper, adipophilin antikoerper, PLIN2 antikoerper, DKFZp469F1012 antikoerper, perilipin-2 antikoerper, zgc:110411 antikoerper, perilipin 2 antikoerper, perilipin 2 L homeolog antikoerper, adipophilin antikoerper, perilipin-2 antikoerper, PLIN2 antikoerper, Plin2 antikoerper, plin2 antikoerper, plin2.L antikoerper, LOC100304704 antikoerper, LOC100380740 antikoerper
    Hintergrund
    Adipophilin / ADRP is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al., for review see e.g. Targett-Adams et al.), inducible by etomoxir. Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (see e.g. Hope et al.).Synonyms: ADRP, Adipose differentiation-related protein
    Gen-ID
    123
    NCBI Accession
    NP_001113
    UniProt
    Q99541
    Pathways
    Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism
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