Desmoplakin Antikörper

Produktnummer ABIN112137

Der Maus Monoklonal anti-Desmoplakin Antikörper (Klon DP-2-17) (ABIN112137) detektiert spezifisch Desmoplakin in WB, IHC (fro) und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Huhn und Rind (Kuh).

Kurzübersicht für Desmoplakin Antikörper (ABIN112137)

Target: Desmoplakin (DSP)

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoplakin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: DP-2-17

Produktdetails anti-Desmoplakin Antikörper

Spezifität: DP 2.17 shows distinct punctate membrane staining of epithelia. It reacts with the rod fragment of Desmoplakin 1 (Mr 250,000 polypeptide of desmosomal plaques). Tested Reactivities on Cultured Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, TR-146. Bovine cells: MDBK, BMGE.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine, Rat, Chicken and Mouse.

Aufreinigung: Protein A Affinity Chromatography

Immunogen: Bovine Desmoplakin 1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen and paraffin embedded sections after microwavetreatment (dilute 1: 10 with PBS pH 7.4). Western blot (Incubation time: 1h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Haltbarkeit: 6 months

Details zu Desmoplakin

Target: Desmoplakin (DSP)

Andere Bezeichnung: Desmoplakin

Hintergrund: Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP

Gen-ID: 1832

NCBI Accession: NP_001008844

UniProt: P15924

Pathways: Cell-Cell Junction Organization