Lamin A/C Antikörper

Produktnummer ABIN112042

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Lamin A/C in WB, IF, IHC (p), FACS und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh) und Hund.

Kurzübersicht für Lamin A/C Antikörper (ABIN112042)

Target: Lamin A/C (LMNA)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 133A2

Produktdetails anti-Lamin A/C Antikörper

Spezifität: The antibody 133A2 recognizes an epitope located between residues 598-611 of Lamin A, therefore it reacts exclusively with Lamin A.

Aufreinigung: Purified

Immunogen: Partially purified recombinant Human Lamin A.

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry. Immunohistochemistry on frozen sections (1: 100 - 1: 200 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent). Immunoblotting (1: 100 - 1: 1000). Flow cytometry (1: 100 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Immunofluorescence protocol - Formaldehyde fixation1. Collect cells from T. c. unit and remove media from petri dish using suction. 2. Wash with 1x PBS and remove. 3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at roomtemperature on an orbital shaker. 4. Remove PFA and incubate in 0. 5% Triton X-IOO in 1x PBS for 5 minutes at roomtemperature. 5. Prepare blocking reagent, this is also the antibody diluent. 6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker. 7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature. 8. Prepare primary antibodies (50μl/coverslip) and moist staining chambers. 9. Wash cells 2x with lx PBS at room temperature and air dry briefly.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: Lamin-A/C (LMNA)

Hintergrund: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32

Molekulargewicht: 74 kDa (Predicted)

Gen-ID: 4000

NCBI Accession: NP_005563

UniProt: P02545

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus