AIMP1 Antikörper

Produktnummer ABIN112011

Dieses Anti-AIMP1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von AIMP1 in WB, EIA, IHC (p) und IHC (fro). Geeignet für Human und Ratte.

Kurzübersicht für AIMP1 Antikörper (ABIN112011)

Target: AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AIMP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 546-2

Produktdetails anti-AIMP1 Antikörper

Spezifität: This antibody reacts with Endothelial-Monocyte-Activating Polypeptide II (EMAP II).

Kreuzreaktivität (Details): Species reactivity (tested):Human, Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Recombinant Human EMAPII. The epitope has not been further characterized.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 10 μg/mL (1/40)Immunohistochemistry on Paraffin Sections: 10 μg/mL (1/40). Microwave pretreatment forantigen retrieval is recommended. Recommended Positive Control: Rat spleen. Has been described to work in ELISA and Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Reconstitute by adding 0.5 mL distilled water.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu AIMP1

Target: AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))

Andere Bezeichnung: SCYE1 / EMAP2

Hintergrund: EMAPII is a proinflammatory cytokine and chemoattractant of macrophages. The 20 kDa mature peptide binds to endothelial cells and leukocytes like macrophages. EMAP II activates endothelial cells, by elevation of cytosolic free calcium concentration, release of von Willebrand factor, induction of tissue factor, and expression of adhesion molecules E-selectin and P-selectin. It further activates resting monocytes by elevating cytosolic free calcium concentration, inducing TNFalpha and tissue factor and stimulating chemotaxis. EMAP II has been shown in a tumor model to sensitize the local tumor vasculature to the action of TNFalpha, rendering resistant tumors sensitive to immunotherapy by TNFalpha.Synonyms: AIMP1, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP-2, EMAP-II, Endothelial monocyte-activating polypeptide 2, Endothelial monocyte-activating polypeptide II, Multisynthase complex auxiliary component p43, Small inducible cytokine subfamily E member 1

Gen-ID: 9255

NCBI Accession: NP_001135887

UniProt: Q12904