Transferrin Receptor Antikörper

Produktnummer ABIN111980

Der Ratte Monoklonal Anti-Transferrin Receptor-Antikörper wurde für FACS und IHC (fro) validiert. Er ist geeignet, Transferrin Receptor in Proben von Maus zu detektieren.

Kurzübersicht für Transferrin Receptor Antikörper (ABIN111980)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Receptor Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ER-MP21

Produktdetails anti-Transferrin Receptor Antikörper

Spezifität: ER-MP21 stains proliferating cells of all types as well as cells taking up iron for special needs, such as late erythroid precursors and some mature macrophages. The antibody inhibits the iron uptake and the proliferation of macrophage precursors from bone marrow and early macrophage precursor cell lines. ER-MP21 recognizes the transferrin receptor but does not compete with transferrin binding. Specific for mouse transferring receptor. The antigen is a 200 kDa protein (non-reducing conditions) consisting of two identical chains. The recognized epitope of the receptor is not located at the transferrin binding site. Antigen Distribution: Macrophage progeny at day 7 in bone marrow cultures, treated in parallel on various days by irradiation (1500 rad 60Co γ-radiation) or addition of ER-MP21 (30 μg/mL final concentration). Cell numbers were quantified using the MTT assay and expressed relative to untreated controls. Relative growth inhibition calculated from data represented in left figure. For mAb- or radiation treated cultures, the number of cell cycles needed to reach the cell number in untreated controls was calculated. Values obtained for irradiated cultures were taken as 100 % inhibition controls and values for ER-MP21 - treated cultures were expressed relative to these (See Figure 1.)

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography

Immunogen: Macrophage precursor cells.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry (IHC) on: Frozen sections: 0.5 μg/mL (1/400)Paraffin sections: does not react on routinely processed paraffin sections. It works also in FACS. Suggested positive control: Mouse spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: CD71 / TFRC

Hintergrund: The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.Synonyms: TfR1, Transferrin receptor protein 1, p90

Gen-ID: 22042

NCBI Accession: NP_035768

UniProt: Q62351

Pathways: Transition Metal Ion Homeostasis