CD31 Antikörper (Biotin)

Produktnummer ABIN111979

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch CD31 in FACS, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus und wurde in 7+ Publikationen erwähnt.

Kurzübersicht für CD31 Antikörper (Biotin) (ABIN111979)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD31 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ER-MP12

Produktdetails anti-CD31 Antikörper (Biotin)

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography.

Immunogen: Mouse macrophage precursor cells. The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions). The antigen expression closely follows that of CD34.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on acetone fixed Frozen Sections (1/1600 = 0.4 μg/mL)and Formalin Fixed Paraffin Sections (1/40 = 15 μg/mL). Antigen Retrieval: Proteinase K. Suggested Positive Control: Mouse spleen. Has been described to work in FACS analysis: 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water (0.6 mg/mL stock solution).

Konzentration: 0.6 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.05 % Kathon as a preservative.

Konservierungsmittel: Kathon CG

Vorsichtsmaßnahmen: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store (in aliquots) at -20 °C.

Referenzen für anti-CD31 Antikörper (Biotin) (ABIN111979)

Mateos, Landeira-Abia, Fafián-Labora, Fernández-Pernas, Lesende-Rodríguez, Fernández-Puente, Fernández-Moreno, Delmiro, Martín, Blanco, Arufe: "iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis." in: Stem cell research & therapy, Vol. 6, pp. 119, (2016) (PubMed).

Morikawa, Kajimura, Nakamura, Hishiki, Nakanishi, Yukutake, Nagahata, Ishikawa, Hattori, Takenouchi, Takahashi, Ishii, Matsubara, Kabe, Uchiyama, Nagata, Gadalla, Snyder, Suematsu: "Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. 1293-8, (2012) (PubMed).

Sakamoto, Geisel, Kim, Wyatt, Sellers, Smiley, Cooper, Russell, Rhoades: "Fibrinogen regulates the cytotoxicity of mycobacterial trehalose dimycolate but is not required for cell recruitment, cytokine response, or control of mycobacterial infection." in: Infection and immunity, Vol. 78, Issue 3, pp. 1004-11, (2010) (PubMed).

Larghero, Venè, Minghelli, Travaini, Morini, Ferrari, Pfeffer, Noonan, Albini, Benelli: "Biological assays and genomic analysis reveal lipoic acid modulation of endothelial cell behavior and gene expression." in: Carcinogenesis, Vol. 28, Issue 5, pp. 1008-20, (2007) (PubMed).

Li, Sainson, Shi, Leek, Harrington, Preusser, Biswas, Turley, Heikamp, Hainfellner, Harris: "Delta-like 4 Notch ligand regulates tumor angiogenesis, improves tumor vascular function, and promotes tumor growth in vivo." in: Cancer research, Vol. 67, Issue 23, pp. 11244-53, (2007) (PubMed).

Burbaeva, Boksha, Tereshkina, Savushkina, Starodubtseva, Turishcheva, Mukaetova-Ladinska: "Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus." in: Neurochemical research, Vol. 32, Issue 9, pp. 1434-44, (2007) (PubMed).

Li, Otsu, Murphy, Raymond: "Developmental decrease in NMDA receptor desensitization associated with shift to synapse and interaction with postsynaptic density-95." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 35, pp. 11244-54, (2003) (PubMed).

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: CD31 / PECAM1

Hintergrund: Anti-PECAM-1 is a general marker for the detection of a major population of haematopoietic stem cells and is useful for the accumulation of early murine macrophage precursors from bone marrow cell suspensions by FACS. An even more precise characterization of such isolated and concentrated precursor cells is achieved when ER-MP12 is combined with other markers like ER-MP20 (anti Ly-6C) or ER-MP58. The ER-MP12 antigen is also expressed by endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

Gen-ID: 10090

UniProt: Q08481

Pathways: Regulation of Actin Filament Polymerization