CD33 Antikörper

Produktnummer ABIN111914

Der Maus Monoklonal Anti-CD33-Antikörper wurde für FACS, IP und IHC (fro) validiert. Er ist geeignet, CD33 in Proben von Human zu detektieren.

Kurzübersicht für CD33 Antikörper (ABIN111914)

Target: CD33

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD33 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MY9

Produktdetails anti-CD33 Antikörper

Spezifität: The CD33 antibody is valuable for distinguishing acute myeloid from lymphoblastic leukaemias. CD33 is expressed on monocytes in peripheral blood. In the bone marrow, CD33 antigen is found on granulocyte and macrophage precursors, and is absent from pluripotent stem cells.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (IHC) on frozen sections: 4 μg/mL (1: 100). FACS: 10 l stock solution to label 10^6 cells. Immunoprecipitation. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, 0.05 % (v/v) Kathon CG, 5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C after reconstitution.

Details zu CD33

Target: CD33

Andere Bezeichnung: CD33 / SIGLEC3

Hintergrund: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is a 67 kDa single chain transmembrane glycoprotein that belongs to the sialoadhesin family.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gen-ID: 945

NCBI Accession: NP_001763

UniProt: P20138