S100A8 Antikörper (C-Term) (Biotin)

Produktnummer ABIN111893

Produktdetails anti-S100A8 Antikörper (Biotin)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A8 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: S 13.67 identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. S 13.67 is useful for the identification of the 8 kDa S100A8 subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. S13.67 detects a very distinct, as yet not characterized epitope of MRP8 (different from the 8-5C2 epitope). Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stains positively. Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-8 increasingly during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14(TNF-alpha/IL-1 alpha induced inflammation of the skin).

Kreuzreaktivität (Details): Species reactivity (tested):Human: MRP8 in stimulated monocytes and macrophages inlate phase or chronic inflammation.Rat, Cow, Pig (TNF-alpha/IL-1 alpha induced inflammation of the skin).

Aufreinigung: Affinity Chromatography

Immunogen: Cultured Human Monocytes. Remarks: The antigen is MRP8, the epitope is suspected in the N- or C-terminal portion of the peptide.

Klon: S13-67

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry. Immunohistochemistry on: Frozen sections: 1 μg/mL (1: 200)Paraffin sections: 5-10 μg/mL (1: 20-1: 50), no pretreatment for antigen retrieval necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 0.5 mL distilled water to make stock solution.

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Andere Bezeichnung: S100A8 / Calgranulin-A / MRP8 (S100A8 Produkte)

Hintergrund: MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A

Gen-ID: 9606

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine