S100A9 Antikörper (Biotin)

Produktnummer ABIN111887

Der Maus Monoklonal Anti-S100A9-Antikörper wurde für FACS, IHC (p), EIA und IHC (fro) validiert. Er ist geeignet, S100A9 in Proben von Human und Rind (Kuh) zu detektieren.

Kurzübersicht für S100A9 Antikörper (Biotin) (ABIN111887)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A9 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: S36-48

Produktdetails anti-S100A9 Antikörper (Biotin)

Spezifität: MRP14, granulocytes, stimulated monocytes and macrophages. Monoclonal antibody BM4026B identifies the Ca2+-binding 14 kDa subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. It is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14 differently depending on the inflammatory status of the disease. Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from all other blood cells. In cultured monocytes, maximum MRP14 expression is found after 3-4 days. Myeloid leukaemic cells have been found to be positive as well. Tissue Sections: MRP14 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP14 increasingly during the early stages of inflammation. A high MRP14 (and low MRP8) expression by macrophages was reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases like chronic rheumatoid arthritis, MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/14 determined by antibody 27E10 (product Cat.-No BM4025).Antigen Distribution

Kreuzreaktivität (Details): Species reactivity (tested):Human.The antibody reacts with Bovine spleen. It does not react with Swine tissues.

Aufreinigung: Affinity Chromatography.

Immunogen: Cultured Human monocytes. Remarks: The antigen is MRP14, the epitope is suspected in the central region of the peptide.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Immunohistochemistry on Frozen Sections: 1 μg/mL (1/200). Immunohistochemistry on Paraffin Sections: 2 μg/mL (1/100). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Also Suitable for Dot Blots and FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Andere Bezeichnung: S100A9 / Calgranulin-B / MRP14

Hintergrund: S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins. S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.Synonyms: CAGB, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, MRP-14, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, S100-A9

Gen-ID: 9606

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine