SFTPD Antikörper (Lectin Domain) (Biotin)

Produktnummer ABIN111842

Der Maus Monoklonal anti-SFTPD Antikörper wird verwendet zum Nachweis von SFTPD in Proben von Human und Ratte. Er wurde validiert für WB, IHC (fro) und EIA.

Kurzübersicht für SFTPD Antikörper (Lectin Domain) (Biotin) (ABIN111842)

Target: SFTPD (Surfactant Protein D (SFTPD))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SFTPD Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: VIF11

Produktdetails anti-SFTPD Antikörper (Biotin)

Bindungsspezifität: Lectin Domain

Spezifität: Antigen Distribution on Tissue Sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.

Aufreinigung: Affinity Chromatography

Immunogen: Purified rat surfactant protein D from bronchoalveolar lavage. Remarks: The antigen epitope is localized in the Lectin domain of SP-D.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (IHC) on Frozen sections (2 μg/mL (1/200) for rat cryosections) andParaffin sections. ELISA. Western Blots. Suggested positive control: Rat lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Andere Bezeichnung: SFTPD

Hintergrund: Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

Gen-ID: 25350

NCBI Accession: NP_037010

UniProt: P35248