Desmoglein 2 Antikörper

Produktnummer ABIN111817

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Desmoglein 2 in WB, IF, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus und Rind (Kuh).

Kurzübersicht für Desmoglein 2 Antikörper (ABIN111817)

Target: Desmoglein 2 (DSG2)

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: DG3-10

Produktdetails anti-Desmoglein 2 Antikörper

Spezifität: This antibody recognizes Desmoglein 1+2 (desmosome-specific cadherins) in desmosome-bearing cells. It does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors Specifically Detected: All epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide Reacting: Mr 165000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope Recognized: Localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other Human epithelial cell lines, Bovine BMGE+H, BMGE-H, MDBK.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine, Rat and Mouse.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: "Band 3" polypeptide of isolated desmosomes from Bovine muzzle epidermis

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting. Immunofluorescence Microscopy. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissue (after microwave treatment). Working Dilution: Dilute at least 1/10 with PBS, pH 7.4, for Immunohistochemicalapplication. Incubation time: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL distilled water.

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Desmoglein 2

Target: Desmoglein 2 (DSG2)

Andere Bezeichnung: Desmoglein-2

Hintergrund: Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 and 2 are calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.Synonyms: CDHF4, CDHF5, DG1, DGI, DSG-1, DSG-2, DSG1, DSG2, Desmoglein1, Desmoglein2, Desmosomal glycoprotein 1, HDGC, Pemphigus foliaceus antigen

Gen-ID: 1829

NCBI Accession: NP_001934

UniProt: Q14126

Pathways: Cell-Cell Junction Organization