His Tag Antikörper

Produktnummer ABIN1112988

Dieses Anti-His Tag-Antikörper ist ein Maus -Antikörper zur Detektion von His Tag in WB, IP und IF. Geeignet für .

Kurzübersicht für His Tag Antikörper (ABIN1112988)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Produktdetails anti-His Tag Antikörper

Sequenz: HHHHHH

Spezifität: Detect over-expressed or recombinant proteins containing the 6 × His epitope tag.

Immunogen: A synthetic peptide 6 × His conjugated to KLH.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB (1:5000), IP (1:100), IF (1:2000). Other applications have not been tested. The optimal dilutions should be determined by end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for 1 year. Or aliquot and store at -80 °C for long term. Avoid repeated freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu His Tag

Target: His Tag

Substanzklasse: Tag

Hintergrund: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Affinity purification using a polyhistidine-tag usually results in relatively pure protein when the recombinant protein is expressed in prokaryotic organisms. Depending on downstream applications including the purification of protein complexes to study protein interactions, purification from higher organisms such as yeasts or other eukaryotes may require a tandem affinity purification using two tags to yield higher purity. The most common polyhistidine tags are formed of six histidine (6xHis tag) residues - preceded by Methionine - which are added at the C-terminal or N-terminal of the protein of interest.