WB (1:10,000-100,000), Immunoprecipitation (1:50-200) . Other applications have not been tested. The optimal dilutions should be determined by end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Konservierungsmittel
Sodium azide, Thimerosal (Merthiolate)
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 4 °C after thawing (1 week). Aliquot and store at -20 °C for long term (at least one year). Avoid repeated freeze and thaw cycles.
Haltbarkeit
12 months
Target
GST
(Glutathione S Transferase (GST))
Andere Bezeichnung
Glutathione S-transferases, GST
Hintergrund
Glutathione S-transferases are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. Genetic polymorphisms in glutathione S-transferase and its altered expression and activity are associated with oxidative DNA damage and also damage of the kidney. Subsequently the individual's risk of cancer susceptibility increases. 1