beta Actin Antikörper (N-Term)

Produktnummer ABIN1112710

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch beta Actin in WB. Er zeigt eine Reaktivität gegenüber Maus, Ratte und Affe.

Kurzübersicht für beta Actin Antikörper (N-Term) (ABIN1112710)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-beta Actin Antikörper

Bindungsspezifität: N-Term

Spezifität: Detect endogenous level of total beta-Actin protein.

Immunogen: A synthetic peptide (conjugated to KLH) corresponding to N-terminal residues of human beta-Actin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB (1:2000) Other applications have not been tested. The optimal dilutions should be determined by end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for 1 year. Avoid repeated freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: beta-Actin

Hintergrund: Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found that approximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation. And arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo. 1 Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets. 2 Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction