Transferrin Receptor Antikörper (FITC)

Produktnummer ABIN1112226

Dieses Anti-Transferrin Receptor-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Transferrin Receptor in FACS und IF. Geeignet für Human.

Kurzübersicht für Transferrin Receptor Antikörper (FITC) (ABIN1112226)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Receptor Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: FG2-12

Produktdetails anti-Transferrin Receptor Antikörper (FITC)

Produktmerkmale: Monoclonal Mouse Anti-Human CD71.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Kommentare:

Fluorescein isothiocyanate (Molecular Probes).

Probennahme: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD71 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 100 µl of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis. MDS (Myelodysplastic Syndrome) Sample from a Human Donor.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: CD71

Hintergrund: Human Transferrin Receptor which is expressed on proliferating lymphoblasts, majority human tumour cell lines, normal peripheral blood monocytes. The CD71 antibody in routine is used along with the glycophorin A to develop a flow-cytometric approach to the evaluation of erythroid and myeloid dysplasia in patients with Myelodysplatic Syndromes (MDS)1. Another of the applications of the CD71 antibody, is the recount of retyculocites2, immature red corpuscles, without nucleus, that contain ribonucleic acid (RNA). peripheral blood, they delay approximately a day in losing their RNA and to be become erythrocytes. Its recount provides information on the production of erythrocytes.

Pathways: Transition Metal Ion Homeostasis