ITGA2 Antikörper (APC)

Produktnummer ABIN1112169

Dieses Anti-ITGA2-Antikörper ist ein Monoklonal-Antikörper zur Detektion von ITGA2 in FACS und IF. Geeignet für Human.

Kurzübersicht für ITGA2 Antikörper (APC) (ABIN1112169)

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reaktivität: Human

Wirt: Bitte anfragen

Klonalität: Monoklonal

Konjugat: Dieser ITGA2 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: TEA1-41

Produktdetails anti-ITGA2 Antikörper (APC)

Produktmerkmale: The TEA1/41 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Kommentare:

Allophycocyanin (Febico, Far East Bio-Tech Co.).

Aufbereitung der Proben:

1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD49b APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Lagerung: 4 °C

Details zu ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Andere Bezeichnung: CD49b

Hintergrund: Human platelet antigen (MW 170 kDa). The antigen is found on platelets, endothelial cells and approximately 50% of monocytes together with activated T cells. The gpla antigen is non- covalently associated with the 130kDa VLA --chain (CD29) in the VLA-2 complex.

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex