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CD2 Antikörper (APC)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD2 in FACS und IF. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN1112110

Kurzübersicht für CD2 Antikörper (APC) (ABIN1112110)

Target

Alle CD2 Antikörper anzeigen
CD2

Reaktivität

  • 185
  • 48
  • 32
  • 16
  • 13
  • 11
  • 8
  • 7
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 149
  • 66
  • 31
  • 3
  • 2
  • 1
  • 1
Maus

Klonalität

  • 188
  • 59
  • 5
Monoklonal

Konjugat

  • 119
  • 26
  • 23
  • 17
  • 9
  • 6
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD2 Antikörper ist konjugiert mit APC

Applikation

  • 187
  • 71
  • 59
  • 49
  • 44
  • 34
  • 32
  • 15
  • 14
  • 13
  • 10
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF)

Klon

TP1-31
  • Produktmerkmale

    Mouse Monoclonal Anti-Human CD2 APC is recommended for use in flow cytometry for identification of peripheral T lymphocytes and 90% of the thymocytes.

    Isotyp

    IgG1
  • Applikationshinweise

    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

    Kommentare

    Allophycocyanin (Febico, Far East Bio-Tech Co.).

    Probennahme

    1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10 P6 P cells). 2. Add 20 µl of CD2 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

    Lagerung

    4 °C
  • Target

    CD2

    Andere Bezeichnung

    CD2

    Hintergrund

    50kDa MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD2 cluster. The antigen is expressed on all peripheral blood T lymphocytes, majority of thymocytes and malignant cells of T cell origin, including T- ALL cells. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen. Common ALL cells do not express CD2 antigen.
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