C5 Antikörper

Produktnummer ABIN111066

Dieses Anti-C5-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von C5 in WB, IHC (fro) und EIA. Geeignet für Human, Schwein, Rind (Kuh), Ziege und Primate.

Kurzübersicht für C5 Antikörper (ABIN111066)

Target: C5 (Complement Component 5 (C5))

Reaktivität: Human, Schwein, Rind (Kuh), Ziege, Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: HCC5b-1 (neo)

Produktdetails anti-C5 Antikörper

Spezifität: HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5! Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000, after C5b activation, an Mr 60 000 cleavage product also reacts (weakly). Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Primates, Bovine, Pig, Goat.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Activated Human Complement Component C5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Immunoblotting (Western blot). Cytological material. Immunohistochemistry on Frozen Sections (1/10). Incubation time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL distilled water.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu C5

Target: C5 (Complement Component 5 (C5))

Andere Bezeichnung: Complement C5

Hintergrund: C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: CPAMD4, Complement Component 5

Gen-ID: 727

NCBI Accession: NP_001726

UniProt: P01031

Pathways: Komplementsystem, Carbohydrate Homeostasis