ENO2/NSE Antikörper

Produktnummer ABIN110966

Produktdetails anti-ENO2/NSE Antikörper

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ENO2/NSE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is specific for Human Neuron Specific Enolase (NSE), a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma. Reacts with a 45 kDa band corresponding to NSE on Immunoblots of brain extracts.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Native NSE protein from Human brain.

Klon: 5G10

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA. Immunohistology on Paraffin Sections: 1/100-1/400. Requires antigen retrieval using heat treatment prior to staining of paraffin sections(Sodium Citrate buffer pH 6.0 is recommended for this purpose). Positive Control: Brain cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS with 0.09 % sodium azide as preservative.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: Neuron Specific Enolase (ENO2 Produkte)

Synonyme: ENO2 antikoerper, DKFZp459B1817 antikoerper, NSE antikoerper, AI837106 antikoerper, D6Ertd375e antikoerper, Eno-2 antikoerper, RNEN3 antikoerper, eno3 antikoerper, wu:fc09h05 antikoerper, zgc:92418 antikoerper, enolase 2 antikoerper, enolase 2 (gamma, neuronal) antikoerper, enolase 2, gamma neuronal antikoerper, enolase 2, gamma, neuronal antikoerper, ENO2 antikoerper, Eno2 antikoerper, eno2 antikoerper

Hintergrund: Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, NSE, Neural enolase

Gen-ID: 2026

NCBI Accession: NP_001966

UniProt: P09104