TJP1 Antikörper (AA 886-995)

Produktnummer ABIN1109613

Dieses Anti-TJP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TJP1 in WB, IF, FACS, IHC (p) und IHC (fro). Geeignet für Human, Maus, Ratte und Hund.

Kurzübersicht für TJP1 Antikörper (AA 886-995) (ABIN1109613)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TJP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-TJP1 Antikörper

Bindungsspezifität: AA 886-995

Spezifität: This antibody recognizes specifically ZO-1 alpha-plus.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat, Dog.

Aufreinigung: Protein A Chromatography

Immunogen: Amino acids 886-995 of human ZO-1

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, 0.1 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C.

Details zu TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Andere Bezeichnung: ZO-1 / Tight Junction Protein 1

Hintergrund: Zona occludens 1 (ZO-1) is an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.Synonyms: Tjp1, Zo1, Zona occludens protein 1, Zonula occludens protein 1

Gen-ID: 7082

NCBI Accession: NP_003248

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization