PRTN3 Antikörper

Produktnummer ABIN1108720

Produktdetails anti-PRTN3 Antikörper

Target: PRTN3 (Proteinase 3 (PRTN3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRTN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Spezifität: Monoclonal antibody WGM2 reacts with human proteinase 3 (PR3), a 30 kDa protein.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Protein G

Klon: WGM2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Andere Bezeichnung: PR3 / C-ANCA (PRTN3 Produkte)

Synonyme: ela2 antikoerper, MGC132250 antikoerper, ACPA antikoerper, AGP7 antikoerper, C-ANCA antikoerper, CANCA antikoerper, MBN antikoerper, MBT antikoerper, NP-4 antikoerper, NP4 antikoerper, P29 antikoerper, PR-3 antikoerper, PR3 antikoerper, mPR3 antikoerper, proteinase 3 L homeolog antikoerper, proteinase 3 antikoerper, prtn3.L antikoerper, PRTN3 antikoerper, Prtn3 antikoerper

Hintergrund: PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (so called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation PR3 can cleave the complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin.Synonyms: AGP7, Leukocyte proteinase 3, MBN, Myeloblastin, NP-4, Neutrophil proteinase 4, P29, PR-3, PRTN3, Proteinase 3, Wegener autoantigen

Gen-ID: 5657

NCBI Accession: NP_002768

UniProt: P24158