PRTN3 Antikörper

Produktnummer ABIN1108719

Produktdetails anti-PRTN3 Antikörper

Target: PRTN3 (Proteinase 3 (PRTN3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRTN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Protein G

Immunogen: A crude granule extract

Klon: PR3-G2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Andere Bezeichnung: PR3 / C-ANCA (PRTN3 Produkte)

Synonyme: ela2 antikoerper, MGC132250 antikoerper, ACPA antikoerper, AGP7 antikoerper, C-ANCA antikoerper, CANCA antikoerper, MBN antikoerper, MBT antikoerper, NP-4 antikoerper, NP4 antikoerper, P29 antikoerper, PR-3 antikoerper, PR3 antikoerper, mPR3 antikoerper, proteinase 3 L homeolog antikoerper, proteinase 3 antikoerper, prtn3.L antikoerper, PRTN3 antikoerper, Prtn3 antikoerper

Hintergrund: PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation, PR3 can cleave the PR3-ANCA complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin.Synonyms: AGP7, Leukocyte proteinase 3, MBN, Myeloblastin, NP-4, Neutrophil proteinase 4, P29, PR-3, PRTN3, Proteinase 3, Wegener autoantigen

UniProt: P24158