Deoxyuridine Triphosphatase (DUT) (Middle Region) Antikörper

Produktnummer ABIN1107021

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in WB, IHC (p) und IP. Geeignet für Human, Ratte und Maus.

Kurzübersicht für Deoxyuridine Triphosphatase (DUT) (Middle Region) Antikörper (ABIN1107021)

Target: Deoxyuridine Triphosphatase (DUT)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails

Bindungsspezifität: Middle Region

Spezifität: This antibody detects DUT in the middle. No cross reactivity with other roteins.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Immunogen affinity purified

Immunogen: Synthetic peptide corresponding to a sequence mapping at the middle region of human dUTPase .Remarks: Sequence is identical to the related rat and ouse sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg himerosal, 0.05 mg NaN3

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Deoxyuridine Triphosphatase (DUT)

Andere Bezeichnung: DUT

Hintergrund: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low ntracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. DUTPase ctivity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and UTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the ytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35 % identity with the E. coli UTPase and 53 % identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial orms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the se of alternative 5-prime exons. Human dUTPase exhibits 92 % identity with rat. Moreover, this nzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.Synonyms: Deoxyuridine 5'-triphosphate nucleotidohydrolase mitochondrial, dUTP pyrophosphatase, dUTPase

Gen-ID: 1854

NCBI Accession: NP_001020419

UniProt: P33316