PDGFRA Antikörper (Extracellular Domain)

Produktnummer ABIN110689

Dieser Maus Monoklonal Antikörper detektiert spezifisch PDGFRA in WB, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PDGFRA Antikörper (Extracellular Domain) (ABIN110689)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDGFRA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 57-20 (35248)

Produktdetails anti-PDGFRA Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: This antibody detects PDGF RECEPTOR ALPHA. The ND50 was determined as 0.2-0.6 μg/mL for blocking 10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of Human WS1 cells.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 0.5-1 μg/mL. Western Blot: 1-2 μg/mL. Immunohistochemistry on paraffin embedded sections: 10-20 μg/mL. Functional assays: The ND50 for this batch was determined as 0.2-0.6 μg/mL for blocking10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 10 % Trehalose as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid multiple freeze/thaw cycles.

Details zu PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Andere Bezeichnung: CD140a / PDGFRA

Hintergrund: PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphidelinked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cellsurface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha

Gen-ID: 5156

NCBI Accession: NP_006197

UniProt: P16234

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Platelet-derived growth Factor Receptor Signaling