PDGFRA Antikörper (Extracellular Domain)

Produktnummer ABIN110689

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PDGFRA in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PDGFRA Antikörper (Extracellular Domain) (ABIN110689)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDGFRA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 57-20 (35248)

Produktdetails anti-PDGFRA Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: This antibody detects PDGF RECEPTOR ALPHA. The ND50 was determined as 0.2-0.6 μg/mL for blocking 10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of Human WS1 cells.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 0.5-1 μg/mL. Western Blot: 1-2 μg/mL. Immunohistochemistry on paraffin embedded sections: 10-20 μg/mL. Functional assays: The ND50 for this batch was determined as 0.2-0.6 μg/mL for blocking10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 10 % Trehalose as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid multiple freeze/thaw cycles.

Details zu PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Andere Bezeichnung: CD140a / PDGFRA

Hintergrund: PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphidelinked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cellsurface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha

Gen-ID: 5156

NCBI Accession: NP_006197

UniProt: P16234

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Platelet-derived growth Factor Receptor Signaling