Collagen Type I Antikörper

Produktnummer ABIN1106741

Der Kaninchen Polyklonal Anti-Collagen Type I-Antikörper wurde für IHC (fro), IF, EIA und DB validiert. Er ist geeignet, Collagen Type I in Proben von Rind (Kuh) zu detektieren.

Kurzübersicht für Collagen Type I Antikörper (ABIN1106741)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type I Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB)

Produktdetails anti-Collagen Type I Antikörper

Spezifität: Specificity was ascertained by direct ELISA using pure Calf Collagen types I and III for coating microplate wells. No binding to calf serum proteins is revealed at simular dilutions of the antibody. Characteristic immunostaining pictures of frozen sections of bovine kidney and skin are produced to certify absence of crossreactivity with basement membrane collagens (types IV and V).

Kreuzreaktivität (Details): Species reactivity (tested):Bovine.
Also reacts to: type III

Aufreinigung: Affinity Chromatography: The affinity purified antibody is obtained by binding to immobilized native calf collagen, mixture of type I and III (the antigens used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.

Immunogen: Native Calf Collagen, mixture of type I and III: Antibodies to Calf Collagen type I and III are raised in rabbits which are numerously immunized with extensively purified native collagen type I and III extracted from bovine skin into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 1 mL distilled water and add preservative if preferred

Konzentration: 0.1 mg/mL

Details zu Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Andere Bezeichnung: Collagen Type I

Hintergrund: It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gen-ID: 282187

NCBI Accession: NP_001029211