This antibody was purified from hybridoma (clone 27-9) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell P3X with Wister rat lymphocyte immunized with E14.5 mouse fetal liver cells.
Junctional adhesion molecules (JAMs) are member of the immunoglobulin superfamily. JAMs play an important role in many cellular processes such as tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is a 37 kDa protein also called JAM-1 or F11R. JAM-A is expressed on endothelial cells, epithelial cells, and also hematopoietic cells such as neutrophils, platelets, monocytes, lymphocyte and erythrocyte. Extracellular domain of JAM-A participates in homophilic interaction stabilizing endothelial or epithelial cell-cell junctions particularly around tight junctions. In addition, JAM-A is important in leukocyte transmigration. Not only homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the β2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to mediate leukocyte transmigration.Synonyms: F11R, JAM-A, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, PAM1, Platelet F11 receptor, Platelet adhesion molecule 1