CD235a/GYPA Antikörper

Produktnummer ABIN1106118

Dieses Anti-CD235a/GYPA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD235a/GYPA in FACS und IHC (fro). Geeignet für Human.

Kurzübersicht für CD235a/GYPA Antikörper (ABIN1106118)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD235a/GYPA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: NaM10-6G4

Produktdetails anti-CD235a/GYPA Antikörper

Spezifität: Clone NAM10-6G4 produces mouse IgG2a immunoglobulins directed against the human CD235a antigen (41 kDa) which is expressed as a cell surface sialoglycoprotein. This antibody may be effectively used for differentiation in acute leukemias, e.g. presence of the GpA antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells. Using the CD235a antibody, a distinction can be made between erythroleukemia and other (acute) leukemias like e.g. myeloid, lymphoid or undifferentiated leukemias [3,4]. CD235a is often used in combination with detection of H-antigen and/or anti-CD36 antibodies for additional characterization of early erythroid cells. Additional immunophenotyping of early erythroleukemias can be performed by detecting myeloid-associated antigens such as CD13, CD14, CD15, CD33 and CD34, to discriminate from lymphoid lineage-associated antigens like CD2, CD7, CD10 and CD19 [6].

Aufreinigung: Affinity chromatography

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.

Details zu CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Andere Bezeichnung: CD235a / GYPA

Hintergrund: Glycophorin A (GpA) antigen (CD235a) is exclusively expressed on human erythroid cells and their progenitors and not on lymphoid or granulocytic progenitor cells and therefore is a very useful marker for detection of the erythroid cell lineage [1,2]. CD235a seems to be absent from erythroid colony forming cells in bone marrow, but is present during maturation from (pro-)erythroid blast cells to mature erythrocytes. CD235a is clinically important in the classification of leukemias.Synonyms: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

Gen-ID: 2993

NCBI Accession: NP_002090

UniProt: P02724

Pathways: Maintenance of Protein Location