nectin-3 Antikörper (Extracellular Domain)

Produktnummer ABIN1105755

Dieses Anti-nectin-3-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von nectin-3 in IP, IHC (fro), IF, EIA und Func. Geeignet für Maus.

Kurzübersicht für nectin-3 Antikörper (Extracellular Domain) (ABIN1105755)

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser nectin-3 Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Klon: 103-A1

Produktdetails anti-nectin-3 Antikörper

Bindungsspezifität: AA 50-400, Extracellular Domain

Spezifität: The monoclonal antibody 103-A1 recognizes Mmouse Nectin-3 at aa 50-400.

Keine Kreuzreaktivität: Human, Ratte (Rattus)

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Protein G

Immunogen: Recombinant mouse nectin-3 extracellular domain (50-400 aa) fused to IgG Fc

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, 0.1 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C.

Details zu nectin-3

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Andere Bezeichnung: CD113 / Nectin 3

Hintergrund: Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calcium-independent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3α, -3β and -3g of which nectin-3α is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.Synonyms: CDw113, Nectin-3, PRR3, PVRL3, Poliovirus receptor-related protein 3

Gen-ID: 58998

NCBI Accession: NP_067470

UniProt: Q9JLB9

Pathways: Cell-Cell Junction Organization