nectin-3 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7043441

Der Kaninchen Polyklonal anti-nectin-3 Antikörper wird verwendet zum Nachweis von nectin-3 in Proben von Maus. Er wurde validiert für WB, IHC, FACS und IF.

Kurzübersicht für nectin-3 Antikörper (Extracellular, N-Term) (ABIN7043441)

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser nectin-3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-nectin-3 Antikörper

Bindungsspezifität: AA 84-97, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Nectin-3/PVRL3

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,rat - identical

Produktmerkmale: Anti-Nectin-3/PVRL3 (extracellular) Antibody (ABIN7043441, ABIN7044696 and ABIN7044697) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Nectin-3 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)ETITQISWEKIHGK, corresponding to amino acid residues 84-97 of mouse Nectin-3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235987)

Blocking Peptide: (ABIN7235987)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu nectin-3

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Andere Bezeichnung: NECTIN3

Hintergrund:

Synonyms: Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113

Description: Nectins are Ca2+-independent immunoglobulin-like cell adhesion molecules that localize at adherens junctions between two neighboring cells. They play an important role in mediating asymmetric homotypic and heterotypic cell-cell adhesions. The nectin family contains four members, nectin-1-4, which are encoded by the PVRL1-4 genes, respectively. Nectin-3 is localized mostly in dendrites and partly in axons and is crucial for the specific interactions between axons and dendrites and proper formation of the synapse1,2. Its structure consists of an extracellular region with three immunoglobulin-like loops, one V type and two C2 types.Nectins binds afadin at its c-terminal region and connects to the actin cytoskeleton. This interaction plays a major role in the formation of various cell-cell junctions in cooperation with or independently of cadherins cell adhesion molecules1,2.Nectins also regulates numerous cellular functions, such as cell movement, proliferation, polarization, survival and differentiation by interacting with various proteins1.

Gen-ID: 58998

NCBI Accession: NM_015480

UniProt: Q9JLB9

Pathways: Cell-Cell Junction Organization