PAI1 Antikörper

Produktnummer ABIN1104210

Dieser Anti-PAI1-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von PAI1 in WB, IHC, ELISA, IHC (p) und Neut. Geeignet für Human.

Kurzübersicht für PAI1 Antikörper (ABIN1104210)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Produktdetails anti-PAI1 Antikörper

Spezifität: Human PAI-1

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human PAI-1.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7483 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPAI-1 (4.5 μg/mL), a concentration of at least 15 μg/mL of this antibody is required. ELISA: To detect hPAI-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PAI-1 (LS-C104564) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPAI-1. Western Blot: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Andere Bezeichnung: SERPINE1 / PAI-1

Hintergrund: Name/Gene ID: SERPINE1
Family: Serine Protease Inhibitor

Synonyms: SERPINE1, PAI1, PLANH1, PAI-1, Serpin E1

Gen-ID: 5054

UniProt: P05121

Pathways: p53 Signalweg, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie, Smooth Muscle Cell Migration