IL-13 Antikörper

Produktnummer ABIN1102453

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch IL-13 in WB, ELISA, IHC, Neut und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für IL-13 Antikörper (ABIN1102453)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IL-13 Antikörper

Marke: IHC-plus™

Spezifität: Human IL-13

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-13.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.12 - 0.15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7417 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-13 (2 ng/mL), a concentration of 0.12-0.15 μg/mL of this antibody is required. ELISA: To detect hIL-13 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-13 (LS-C104340) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-13. Western Blot: To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Andere Bezeichnung: IL13

Hintergrund: Name/Gene ID: IL13
Family: Hormone

Synonyms: IL13, ALRH, BHR1, Interleukin-13, NC30, p600, IL-13, Interleukin 13

Gen-ID: 3596

UniProt: P35225

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport