IL-10 Antikörper
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- Target Alle IL-10 (IL10) Antikörper anzeigen
- IL-10 (IL10) (Interleukin 10 (IL10))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser IL-10 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human IL-10
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human IL-10.
Type of Immunogen: Cell extract - Isotyp
- IgG
- Top Product
- Discover our top product IL10 Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7432 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-10 (1 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA: To detect hIL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (LS-C104321) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Sterile water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- IL-10 (IL10) (Interleukin 10 (IL10))
- Andere Bezeichnung
- IL10 (IL10 Produkte)
- Synonyme
- CSIF antikoerper, GVHDS antikoerper, IL-10 antikoerper, IL10A antikoerper, TGIF antikoerper, Il-10 antikoerper, IL10X antikoerper, interleukin 10 antikoerper, IL10 antikoerper, Il10 antikoerper
- Hintergrund
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Name/Gene ID: IL10
Family: Hormone
Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF - Gen-ID
- 3586
- UniProt
- P22301
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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