IGF2 Antikörper

Produktnummer ABIN1102257

Der Kaninchen Polyklonal anti-IGF2 Antikörper (ABIN1102257) detektiert spezifisch IGF2 in WB, IHC, ELISA, IHC (p) und Neut. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für IGF2 Antikörper (ABIN1102257)

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IGF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Produktdetails anti-IGF2 Antikörper

Spezifität: Human IGF-II

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IGF-II.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (3 - 5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7415 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1-4.6 μg/mL of this antibody is required. ELISA: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-II (LS-C104384) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IGF2

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Andere Bezeichnung: IGF2

Hintergrund: Name/Gene ID: IGF2
Family: Hormone

Synonyms: IGF2, C11orf43, Insulin-like growth factor II, IGF-II, PP9974, Somatomedin-A

Gen-ID: 3481

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation