FGF2 Antikörper

Produktnummer ABIN1101523

Produktdetails anti-FGF2 Antikörper

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FGF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Marke: IHC-plus™

Spezifität: Human FGF-basic

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human FGF-basic.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (0.25 - 0.4 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7426 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-basic (0.3 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (LS-C104313) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-basic. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: FGF2 / BFGF (FGF2 Produkte)

Synonyme: BFGF antikoerper, FGF-2 antikoerper, FGFB antikoerper, HBGF-2 antikoerper, Fgf-2 antikoerper, Fgfb antikoerper, bFGF antikoerper, fibroblast growth factor 2 antikoerper, fibroblast growth factor 2 (basic) antikoerper, FGF2 antikoerper, Fgf2 antikoerper, fgf2 antikoerper

Hintergrund: Name/Gene ID: FGF2
Family: HBGF

Synonyms: FGF2, Basic fibroblast growth factor, FGFB, Fibroblast growth factor 2, FGF-2, Prostatropin, BFGF, HBGF-2

Gen-ID: 2247

UniProt: P09038

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine