alpha Defensin 1 Antikörper

Produktnummer ABIN1101107

Der Ziege Polyklonal Anti-alpha Defensin 1-Antikörper wurde für WB, IHC, ELISA, Neut und IHC (p) validiert. Er ist geeignet, alpha Defensin 1 in Proben von Human zu detektieren.

Kurzübersicht für alpha Defensin 1 Antikörper (ABIN1101107)

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser alpha Defensin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-alpha Defensin 1 Antikörper

Marke: IHC-plus™

Spezifität: Human NP-1

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human NP-1.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.4 - 0.6 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7416 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human NP-1 (100 ng/mL), a concentration of 0.40-0.60 μg/mL of this antibody is required. ELISA: To detect hNP-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NP-1 (LS-C104516) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNP-1. Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu alpha Defensin 1

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Andere Bezeichnung: DEFA1 / HP1

Hintergrund: Name/Gene ID: DEFA1

Synonyms: DEFA1, DEFA2, Defensin, alpha 2, HP-1, HP1, Myeloid-related sequence, MRS, DEF1, Defensin, alpha 1, HNP-1, Neutrophil defensin 1

Gen-ID: 1667

UniProt: P59665

Pathways: Cellular Response to Molecule of Bacterial Origin