CXCL12 Antikörper

Produktnummer ABIN1101028

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CXCL12 in WB, IHC, ELISA, IHC (p) und Neut. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CXCL12 Antikörper (ABIN1101028)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Produktdetails anti-CXCL12 Antikörper

Marke: IHC-plus™

Spezifität: Human SDF-1alpha

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human SDF-1alpha.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (2.5 μg/mL), Neut (2 - 4 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7489 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human SDF-1alpha (100 ng/mL), a concentration of 2-4 μg/mL of this antibody is required. ELISA: To detect Human SDF-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SDF-1alpha (LS-C104650) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF-1alpha. Western Blot: To detect Human SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: SDF-1 / CXCL12

Hintergrund: Name/Gene ID: CXCL12
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL12, C-X-C motif chemokine 12, HIRH, IRH, SDF-1, SDF-1b, SDF1A, SDF1B, TPAR1, TLSF-a, TLSF-b, SDF-1a, SDF1, Sdf1alpha, Stromal cell-derived factor 1, TLSF, HSDF-1, PBSF, SCYB12

Gen-ID: 6387

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling