A2BP1 Antikörper (AA 394-646)

Produktnummer ABIN108467

Produktdetails anti-A2BP1 Antikörper

Target: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Bindungsspezifität: AA 394-646

Reaktivität: Chlamydomonas reinhardtii

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser A2BP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: Chlamydomonas reinhardtii

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 122 / 120 kDa

Aufreinigung: serum

Immunogen: residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

Anwendungsinformationen

Applikationshinweise: 1: 1000 - 1: 5000 with standard ECL (WB),

Kommentare:

This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-A2BP1 Antikörper (ABIN108467)

Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

Details zu A2BP1

Target: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Andere Bezeichnung: FOX1 (A2BP1 Produkte)

Hintergrund: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

Molekulargewicht: expected: 122 kDa, apparent: 120 kDa

Gen-ID: 394646

UniProt: A8IZT9