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Uricase (ABC3735) Antikörper

Der Ziege Polyklonal Anti--Antikörper wurde für ELISA, IP und WB validiert. Er ist geeignet, in Proben von Bacillus zu detektieren.
Rockland
Produktnummer ABIN108080
Hersteller Produkt- Nr.: 200-101-092-0100

Kurzübersicht für Uricase (ABC3735) Antikörper (ABIN108080)

Target

Uricase (ABC3735)

Reaktivität

Bacillus

Wirt

  • 3
Ziege

Klonalität

  • 3
Polyklonal

Konjugat

  • 2
  • 1
Unkonjugiert

Applikation

ELISA, Immunoprecipitation (IP), Western Blotting (WB)
  • Hersteller Produkt- Nr.

    200-101-092-0100

    Hersteller

    Rockland

    Verwendungszweck

    Uricase Antibody

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].

    Produktmerkmale

    Synonyms: goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase

    Aufreinigung

    Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogen

    Immunogen: Uricase [Bacillus species]

    Immunogen Type: Native Protein

    Isotyp

    IgG
  • Applikationshinweise

    Application Note: Uricase Antibody has been tested by western blot and is assayed against 1.0 μg of Uricase [Bacillus species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:1,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:1,000 - 1:2,500

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    10.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Target

    Uricase (ABC3735)

    Andere Bezeichnung

    ABC3735

    Substanzklasse

    Dinucleotide

    Hintergrund

    Background: Uricase, or urate oxidase, catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. Urate oxidase is found in nearly all organisms, from bacteria to mammals, and plays different metabolic roles, depending on its host organism. Humans are the only animal that are unable to break down uric acid to allantoin. This is because humans do not have the necessary enzyme uricase. Humans do have a gene for urate oxidase, but it is nonfunctional. Thus uric acid is the end product of animal products in humans. This leads to an increased possibility of an accumulation of uric acid in the body when animal products are eaten. Excessive concentration of uric acid in the blood stream leads to gout. It has been proposed that the loss of urate oxidase gene expression has been advantageous to primates, since uric acid is a powerful antioxidant and scavenger of singlet oxygen and radicals. Its presence provides the body with protection from oxidative damage, thus prolonging life and decreasing age-specific cancer rates.

    Gen-ID

    3202700

    NCBI Accession

    YP_177228

    UniProt

    Q5WBJ3
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