Trypsinogen Antikörper

Produktnummer ABIN107877

Hersteller Produkt- Nr.: 200-4180-0100

Der Kaninchen Polyklonal Anti-Trypsinogen-Antikörper wurde für WB, ELISA und DB validiert. Er ist geeignet, Trypsinogen in Proben von Rind (Kuh) zu detektieren.

Kurzübersicht für Trypsinogen Antikörper (ABIN107877)

Target: Trypsinogen

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Trypsinogen Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB)

Produktdetails anti-Trypsinogen Antikörper

Hersteller Produkt- Nr.: 200-4180-0100

Hersteller: Rockland

Verwendungszweck: Trypsinogen Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].

Produktmerkmale: Synonyms: rabbit anti-Trypsinogen Antibody, Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody

Aufreinigung: Anti-Trypsinogen is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Trypsinogen [Bovine Pancreas]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Trypsinogen Antibody has been tested by ELIDA and dot blot and is assayed against 1.0 μg of Trypsinogen [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,300 to 1:8,400 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Trypsinogen

Target: Trypsinogen

Hintergrund: Background: Trypsinogen, is a precursor to trypsin and a digestive enzyme. It functions as storage of an inactive form of trypsin and kept in the pancreas then released in significant amount, along with other enzymes, when required for protein digestion. Serum trypsinogen is measured using a blood test. Diseases associated with Trypsinogen high levels or mutations are acute pancreatitis and cystic fibrosis. Anti-Trypsinogen Antibody can be useful for researchers interested in studying the serum or pancreas.

Gen-ID: 282603

UniProt: Q29463