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Trypsinogen Antikörper (Biotin)

Reaktivität: Rind (Kuh) WB, ELISA Wirt: Kaninchen Polyclonal Biotin
Produktnummer ABIN1607813
  • Target Alle Trypsinogen Produkte
    Trypsinogen
    Reaktivität
    Rind (Kuh)
    Wirt
    • 5
    Kaninchen
    Klonalität
    • 5
    Polyklonal
    Konjugat
    • 2
    • 2
    • 1
    Dieser Trypsinogen Antikörper ist konjugiert mit Biotin
    Applikation
    • 5
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA
    Kreuzreaktivität (Details)
    Cross reactivity against Trypsinogen from other sources may occur but has not been specifically determined.
    Reinheit
    Anti-Trypsinogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].
    Endotoxin-Niveau
    Low Endotoxin : No
    Immunogen
    Trypsinogen [Bovine Pancreas]
    Immunogen Type: Native Protein
    Isotyp
    IgG
  • Applikationshinweise
    Anti-Trypsinogen antibody has been assayed against 1.0 µg of Trypsinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:25.000
    Western Blot Dilution: 1:500 - 1:3.000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handhabung
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Haltbarkeit
    Expiration date is one (1) year from date of opening.
  • Target
    Trypsinogen
    Abstract
    Trypsinogen Produkte
    Synonyme
    PRSS2 antikoerper, LOC397853 antikoerper, Trypsin antikoerper, LOC100302368 antikoerper, LOC446096 antikoerper, tryp antikoerper, tryl antikoerper, prss2a antikoerper, wu:fb61f11 antikoerper, protease, serine 3 antikoerper, protease, serine 1 L homeolog antikoerper, trypsinogen antikoerper, protease, serine, 59, tandem duplicate 1 antikoerper, PRSS3 antikoerper, prss1.L antikoerper, LOC100302368 antikoerper, LOC446096 antikoerper, tryp antikoerper, prss59.1 antikoerper, LOC100379576 antikoerper
    Hintergrund
    Trypsinogen is the precursor form or zymogen of the pancreatic enzyme trypsin. It is found in pancreatic juice, along with amylase, lipase, and chymotrypsinogen. It is activated by enteropeptidase, which is found in the intestinal mucosa, to form trypsin. Once activated, the trypsin can activate more trypsinogen into trypsin. Trypsin cleaves the peptide bond on the carboxyl side of basic amino acids such as arginine and lysine. Anti-Trypsinogen antibody is ideal investigator involved in cytokines and growth factor research. Synonyms: Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody
    Gen-ID
    282603
    UniProt
    Q29463
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